Coding

Part:BBa_K3031002:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-13)


CwA (194aa) - Truncated cell wall anchoring protein from Lactobacillus plantarum truncated sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of illegal restriction sites.

The 194 amino acids that make up this truncated protein was obtained from the whole protein sequence and was counted backwards from the N-terminal side of the conserved LPxTG motif and low complexity region found on the whole cell wall anchor protein (part BBa_K3031001).

The amino acid sequence for the truncated protein was then derived by counting backwards from the beginning of the conserved LPxTG motif and low complexity region. The result is a protein containing of length 225 amino acids containing 194 amino acids of the Lp_2578 protein + LPxTG anchoring region + low complexity region of 24 amino acids.


Source

The full protein sequence was obtained from the Lp_2578 gene annotation page on NCBI: https://www.ncbi.nlm.nih.gov/gene/?term=lp_2578.

Information for truncated protein size was obtained from the paper "Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells" Moberglisen et al., (2015). The amino acid sequence for the truncated protein was then derived by counting backwards from the beginning of the conserved LPxTG motif and low complexity region. The result is a protein containing of length 225 amino acids containing 194 amino acids of the Lp_2578 protein + LPxTG anchoring region + low complexity region of 24 amino acids.

References

"Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells" Moberglisen et al., (2015).